Detailed information of TA system TA092364

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4717350..4718185
• Replicon: chromosome
• Accession: NZ_CP025903
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: CXV43_RS23205
• Protein ID: WP_000854821.1
• Coordinates: 4717350..4717727 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: CXV43_RS23210
• Protein ID: WP_001285610.1
• Coordinates: 4717817..4718185 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS23175	4712488..4714119	-	1632	WP_001216477.1	sensor histidine kinase	-
CXV43_RS23180	4714345..4715042	-	698	Protein_4488	IS1 family transposase	-
CXV43_RS23185	4715094..4715255	-	162	Protein_4489	transcriptional regulator	-
CXV43_RS23190	4716004..4716846	-	843	WP_001280493.1	DUF4942 domain-containing protein	-
CXV43_RS23195	4716931..4717128	-	198	WP_085949090.1	DUF957 domain-containing protein	-
CXV43_RS23200	4717156..4717353	-	198	Protein_4492	hypothetical protein	-
CXV43_RS23205	4717350..4717727	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CXV43_RS23210	4717817..4718185	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXV43_RS23215	4718265..4718486	-	222	WP_000692350.1	DUF987 domain-containing protein	-
CXV43_RS23220	4718573..4719049	-	477	WP_001384108.1	RadC family protein	-
CXV43_RS23225	4719065..4719235	-	171	Protein_4497	antirestriction protein	-
CXV43_RS23230	4719237..4720004	-	768	Protein_4498	autotransporter adhesin Ag43	-
CXV43_RS23235	4720376..4721248	-	873	WP_001413917.1	GTPase family protein	-
CXV43_RS23240	4721358..4722377	-	1020	WP_001364486.1	replication regulatory protein RepB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4714767..4715042	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T92364 WP_000854821.1 NZ_CP025903:c4717727-4717350 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T92364 NZ_CP025903:c4717727-4717350 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT92364 WP_001285610.1 NZ_CP025903:c4718185-4717817 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT92364 NZ_CP025903:c4718185-4717817 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54