Detailed information of TA system TA092349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1864373..1864899
• Replicon: chromosome
• Accession: NZ_CP025903
• Organism: Escherichia coli strain 300709

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: CXV43_RS09255
• Protein ID: WP_000323025.1
• Coordinates: 1864373..1864660 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: CXV43_RS09260
• Protein ID: WP_000534858.1
• Coordinates: 1864660..1864899 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV43_RS09210	1859397..1859612	-	216	WP_000839590.1	phage lysis protein EssD	-
CXV43_RS25455	1859832..1860002	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
CXV43_RS09215	1860366..1860581	-	216	WP_000066484.1	cold shock-like protein CspB	-
CXV43_RS09220	1860882..1861094	+	213	WP_000087756.1	cold shock-like protein CspF	-
CXV43_RS09225	1861149..1861238	+	90	WP_120795389.1	hypothetical protein	-
CXV43_RS09230	1861516..1862268	-	753	WP_001047135.1	antitermination protein	-
CXV43_RS09235	1862282..1863331	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
CXV43_RS09240	1863333..1863611	-	279	WP_012304870.1	hypothetical protein	-
CXV43_RS09245	1863678..1863929	-	252	WP_000980994.1	hypothetical protein	-
CXV43_RS09250	1864146..1864301	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
CXV43_RS09255	1864373..1864660	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
CXV43_RS09260	1864660..1864899	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
CXV43_RS09265	1864924..1865229	+	306	WP_001326990.1	hypothetical protein	-
CXV43_RS09270	1865432..1865764	+	333	WP_001301033.1	protein FlxA	-
CXV43_RS09275	1866201..1867514	-	1314	WP_021558774.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1835274..1874842	39568

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T92349 WP_000323025.1 NZ_CP025903:c1864660-1864373 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T92349 NZ_CP025903:c1864660-1864373 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT92349 WP_000534858.1 NZ_CP025903:c1864899-1864660 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT92349 NZ_CP025903:c1864899-1864660 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8