Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1448813..1449451 Replicon chromosome
Accession NZ_CP025892
Organism Escherichia coli strain 503025

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CXX03_RS07235 Protein ID WP_000813794.1
Coordinates 1448813..1448989 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CXX03_RS07240 Protein ID WP_023485917.1
Coordinates 1449035..1449451 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX03_RS07215 1444432..1445646 - 1215 WP_001307190.1 BenE family transporter YdcO -
CXX03_RS07220 1445699..1446235 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
CXX03_RS07225 1446308..1448269 + 1962 WP_023485916.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CXX03_RS07230 1448361..1448591 - 231 WP_000494244.1 YncJ family protein -
CXX03_RS07235 1448813..1448989 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CXX03_RS07240 1449035..1449451 + 417 WP_023485917.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CXX03_RS07245 1449530..1450936 + 1407 WP_001296748.1 PLP-dependent aminotransferase family protein -
CXX03_RS07250 1451181..1452326 + 1146 WP_001296759.1 ABC transporter substrate-binding protein -
CXX03_RS07255 1452344..1453357 + 1014 WP_000220416.1 ABC transporter ATP-binding protein -
CXX03_RS07260 1453358..1454299 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - vgrG/tssI / espR1 / fimA / sodB 1352674..1742976 390302
- flank IS/Tn - - 1443127..1444392 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T92322 WP_000813794.1 NZ_CP025892:1448813-1448989 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T92322 NZ_CP025892:1448813-1448989 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15236.59 Da        Isoelectric Point: 4.7386

>AT92322 WP_023485917.1 NZ_CP025892:1449035-1449451 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSSLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT92322 NZ_CP025892:1449035-1449451 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGTCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References