Detailed information of TA system TA092321

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1037014..1037858
• Replicon: chromosome
• Accession: NZ_CP025892
• Organism: Escherichia coli strain 503025

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: CXX03_RS05080
• Protein ID: WP_000854686.1
• Coordinates: 1037475..1037858 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: CXX03_RS05075
• Protein ID: WP_001285602.1
• Coordinates: 1037014..1037394 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX03_RS05035	1033263..1033718	+	456	WP_001504120.1	hypothetical protein	-
CXX03_RS05040	1033797..1034030	+	234	WP_001119717.1	DUF905 family protein	-
CXX03_RS05050	1034130..1034948	+	819	WP_001234732.1	DUF945 domain-containing protein	-
CXX03_RS05055	1035040..1035525	+	486	WP_000214307.1	antirestriction protein	-
CXX03_RS05060	1035541..1036017	+	477	WP_001186726.1	RadC family protein	-
CXX03_RS05065	1036080..1036301	+	222	WP_000692298.1	DUF987 domain-containing protein	-
CXX03_RS05070	1036320..1037003	+	684	WP_000086768.1	hypothetical protein	-
CXX03_RS05075	1037014..1037394	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXX03_RS05080	1037475..1037858	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
CXX03_RS05085	1037855..1038343	+	489	WP_001054233.1	hypothetical protein	-
CXX03_RS05090	1038360..1038557	+	198	WP_000839253.1	DUF957 domain-containing protein	-
CXX03_RS05095	1038642..1039484	+	843	WP_001431817.1	DUF4942 domain-containing protein	-
CXX03_RS05105	1039955..1040893	+	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
CXX03_RS05110	1040948..1041685	+	738	WP_000283664.1	zinc-binding phosphatase	-
CXX03_RS05115	1041709..1042263	+	555	WP_001001902.1	molecular chaperone YcdY	-
CXX03_RS05120	1042365..1042856	+	492	WP_001297187.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T92321 WP_000854686.1 NZ_CP025892:1037475-1037858 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T92321 NZ_CP025892:1037475-1037858 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT92321 WP_001285602.1 NZ_CP025892:1037014-1037394 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT92321 NZ_CP025892:1037014-1037394 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG