Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4138722..4139219 Replicon chromosome
Accession NZ_CP025881
Organism Escherichia coli strain 503440

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXX05_RS20665 Protein ID WP_001173343.1
Coordinates 4139046..4139219 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXX05_RS20660 Protein ID WP_000398619.1
Coordinates 4138722..4139018 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX05_RS20640 4135453..4136172 - 720 WP_000611288.1 two-component system response regulator DcuR -
CXX05_RS20645 4136169..4137800 - 1632 WP_001216460.1 sensor histidine kinase -
CXX05_RS20650 4137981..4138211 + 231 WP_000371699.1 (4Fe-4S)-binding protein -
CXX05_RS20655 4138223..4138495 + 273 WP_000405647.1 N-acetyltransferase -
CXX05_RS20660 4138722..4139018 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXX05_RS20665 4139046..4139219 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXX05_RS20670 4139338..4140855 - 1518 WP_001295063.1 lysine--tRNA ligase -
CXX05_RS20675 4141092..4142453 - 1362 WP_050560790.1 dipeptide/tripeptide permease DtpC -
CXX05_RS20680 4142503..4143200 + 698 Protein_3941 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92312 WP_001173343.1 NZ_CP025881:4139046-4139219 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92312 NZ_CP025881:4139046-4139219 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92312 WP_000398619.1 NZ_CP025881:4138722-4139018 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92312 NZ_CP025881:4138722-4139018 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References