Detailed information of TA system TA092295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1096853..1097491
• Replicon: chromosome
• Accession: NZ_CP025881
• Organism: Escherichia coli strain 503440

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CXX05_RS05455
• Protein ID: WP_000813794.1
• Coordinates: 1096853..1097029 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CXX05_RS05460
• Protein ID: WP_023485917.1
• Coordinates: 1097075..1097491 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX05_RS05435	1092472..1093686	-	1215	WP_001307190.1	BenE family transporter YdcO	-
CXX05_RS05440	1093739..1094275	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CXX05_RS05445	1094348..1096309	+	1962	WP_023485916.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CXX05_RS05450	1096401..1096631	-	231	WP_000494244.1	YncJ family protein	-
CXX05_RS05455	1096853..1097029	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CXX05_RS05460	1097075..1097491	+	417	WP_023485917.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CXX05_RS05465	1097570..1098976	+	1407	WP_001296748.1	PLP-dependent aminotransferase family protein	-
CXX05_RS05470	1099221..1100366	+	1146	WP_001296759.1	ABC transporter substrate-binding protein	-
CXX05_RS05475	1100384..1101397	+	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
CXX05_RS05480	1101398..1102339	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	vgrG/tssI / espR1 / fimA / sodB	969395..1369212	399817
	-	flank	IS/Tn	-	-	1091167..1092432	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T92295 WP_000813794.1 NZ_CP025881:1096853-1097029 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T92295 NZ_CP025881:1096853-1097029 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15236.59 Da
• Isoelectric Point: 4.7386

>AT92295 WP_023485917.1 NZ_CP025881:1097075-1097491 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSSLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT92295 NZ_CP025881:1097075-1097491 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGTCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB