Detailed information of TA system TA092289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3293524..3294162
• Replicon: chromosome
• Accession: NZ_CP025869
• Organism: Escherichia coli strain 503829

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CXX08_RS16330
• Protein ID: WP_000813794.1
• Coordinates: 3293986..3294162 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CXX08_RS16325
• Protein ID: WP_023485917.1
• Coordinates: 3293524..3293940 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX08_RS16305	3288676..3289617	-	942	WP_001251315.1	ABC transporter permease	-
CXX08_RS16310	3289618..3290631	-	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
CXX08_RS16315	3290649..3291794	-	1146	WP_001296759.1	ABC transporter substrate-binding protein	-
CXX08_RS16320	3292039..3293445	-	1407	WP_001296748.1	PLP-dependent aminotransferase family protein	-
CXX08_RS16325	3293524..3293940	-	417	WP_023485917.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CXX08_RS16330	3293986..3294162	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CXX08_RS16335	3294384..3294614	+	231	WP_000494244.1	YncJ family protein	-
CXX08_RS16340	3294706..3296667	-	1962	WP_023485916.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CXX08_RS16345	3296740..3297276	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CXX08_RS16350	3297329..3298543	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	fimA / espR1 / vgrG/tssI	3024179..3416204	392025
	-	flank	IS/Tn	-	-	3298583..3299848	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T92289 WP_000813794.1 NZ_CP025869:c3294162-3293986 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T92289 NZ_CP025869:c3294162-3293986 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15236.59 Da
• Isoelectric Point: 4.7386

>AT92289 WP_023485917.1 NZ_CP025869:c3293940-3293524 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSSLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT92289 NZ_CP025869:c3293940-3293524 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGTCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB