Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2117813..2118438 Replicon chromosome
Accession NZ_CP025869
Organism Escherichia coli strain 503829

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CXX08_RS10365 Protein ID WP_000911329.1
Coordinates 2118040..2118438 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CXX08_RS10360 Protein ID WP_000450524.1
Coordinates 2117813..2118040 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX08_RS10335 2113614..2114084 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CXX08_RS10340 2114084..2114656 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CXX08_RS10345 2114802..2115680 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CXX08_RS10350 2115697..2116731 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CXX08_RS10355 2116944..2117657 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CXX08_RS10360 2117813..2118040 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CXX08_RS10365 2118040..2118438 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CXX08_RS10370 2118585..2119448 + 864 WP_001267507.1 neutral zinc metallopeptidase -
CXX08_RS10375 2119463..2121478 + 2016 WP_000829332.1 tRNA cytosine(34) acetyltransferase TmcA -
CXX08_RS10380 2121552..2122250 + 699 WP_000679812.1 esterase -
CXX08_RS10385 2122360..2122560 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2064260..2154564 90304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T92278 WP_000911329.1 NZ_CP025869:2118040-2118438 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T92278 NZ_CP025869:2118040-2118438 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT92278 WP_000450524.1 NZ_CP025869:2117813-2118040 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT92278 NZ_CP025869:2117813-2118040 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References