Detailed information of TA system TA092270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 135128..136811
• Replicon: chromosome
• Accession: NZ_CP025869
• Organism: Escherichia coli strain 503829

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: CXX08_RS00665
• Protein ID: WP_001717213.1
• Coordinates: 135894..136811 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: CXX08_RS00660
• Protein ID: WP_000157137.1
• Coordinates: 135128..135901 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX08_RS00640	130907..131206	-	300	Protein_122	type 1 fimbria chaperone FimC	-
CXX08_RS00645	131206..133080	+	1875	WP_097310396.1	protease Lon-related BREX system protein BrxL	-
CXX08_RS00650	133199..134548	+	1350	WP_000766444.1	phospholipase	-
CXX08_RS00655	134724..135044	-	321	Protein_125	IS3 family transposase	-
CXX08_RS00660	135128..135901	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
CXX08_RS00665	135894..136811	+	918	WP_001717213.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CXX08_RS00670	137325..137987	-	663	WP_001717211.1	hypothetical protein	-
CXX08_RS00675	137991..138701	-	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
CXX08_RS00685	139582..139785	+	204	WP_021536027.1	AlpA family transcriptional regulator	-
CXX08_RS00690	139969..140283	+	315	WP_001090932.1	hypothetical protein	-
CXX08_RS00695	140363..140659	+	297	WP_000471918.1	hypothetical protein	-
CXX08_RS00700	140712..140948	+	237	WP_000182398.1	hypothetical protein	-
CXX08_RS00705	140988..141338	+	351	WP_000658726.1	hypothetical protein	-
CXX08_RS00710	141408..141647	+	240	WP_001398332.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	135128..165759	30631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 35020.14 Da
• Isoelectric Point: 4.8429

>T92270 WP_001717213.1 NZ_CP025869:135894-136811 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYDGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T92270 NZ_CP025869:135894-136811 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACTTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGAAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGGACACTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGATGGCAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACACAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGTGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCACGTTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCACGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGATGTCATTTAAACGCGGCGAGCCAGACTGGAATTTAGCGCCAGAGCCCCA
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATGTCGGAGGAAAAGCGCGCGCAGTCACTGG
AGAAACTAGAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT92270 WP_000157137.1 NZ_CP025869:135128-135901 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT92270 NZ_CP025869:135128-135901 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGCCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2