Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2077995..2078492 Replicon chromosome
Accession NZ_CP025865
Organism Escherichia coli strain 504211

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXX09_RS10205 Protein ID WP_001173343.1
Coordinates 2077995..2078168 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXX09_RS10210 Protein ID WP_000398619.1
Coordinates 2078196..2078492 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX09_RS10190 2074014..2074711 - 698 Protein_1954 IS1 family transposase -
CXX09_RS10195 2074761..2076122 + 1362 WP_050560790.1 dipeptide/tripeptide permease DtpC -
CXX09_RS10200 2076359..2077876 + 1518 WP_001295063.1 lysine--tRNA ligase -
CXX09_RS10205 2077995..2078168 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXX09_RS10210 2078196..2078492 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXX09_RS10215 2078719..2078991 - 273 WP_000405647.1 N-acetyltransferase -
CXX09_RS10220 2079003..2079233 - 231 WP_000371699.1 (4Fe-4S)-binding protein -
CXX09_RS10225 2079414..2081045 + 1632 WP_001216460.1 sensor histidine kinase -
CXX09_RS10230 2081042..2081761 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92253 WP_001173343.1 NZ_CP025865:c2078168-2077995 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92253 NZ_CP025865:c2078168-2077995 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92253 WP_000398619.1 NZ_CP025865:c2078492-2078196 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92253 NZ_CP025865:c2078492-2078196 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References