Detailed information of TA system TA092251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 1897012..1898695
• Replicon: chromosome
• Accession: NZ_CP025865
• Organism: Escherichia coli strain 504211

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: CXX09_RS09290
• Protein ID: WP_001717213.1
• Coordinates: 1897778..1898695 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: CXX09_RS09285
• Protein ID: WP_000157137.1
• Coordinates: 1897012..1897785 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX09_RS09265	1892791..1893090	-	300	Protein_1779	type 1 fimbria chaperone FimC	-
CXX09_RS09270	1893090..1894964	+	1875	WP_097310396.1	protease Lon-related BREX system protein BrxL	-
CXX09_RS09275	1895083..1896432	+	1350	WP_000766444.1	phospholipase	-
CXX09_RS09280	1896608..1896928	-	321	Protein_1782	IS3 family transposase	-
CXX09_RS09285	1897012..1897785	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
CXX09_RS09290	1897778..1898695	+	918	WP_001717213.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CXX09_RS09295	1899209..1899871	-	663	WP_001717211.1	hypothetical protein	-
CXX09_RS09300	1899875..1900585	-	711	WP_001219054.1	integrase arm-type DNA-binding domain-containing protein	-
CXX09_RS09310	1901466..1901669	+	204	WP_021536027.1	AlpA family transcriptional regulator	-
CXX09_RS09315	1901853..1902167	+	315	WP_001090932.1	hypothetical protein	-
CXX09_RS09320	1902247..1902543	+	297	WP_000471918.1	hypothetical protein	-
CXX09_RS09325	1902596..1902832	+	237	WP_000182398.1	hypothetical protein	-
CXX09_RS09330	1902872..1903222	+	351	WP_000658726.1	hypothetical protein	-
CXX09_RS09335	1903292..1903531	+	240	WP_001398332.1	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1897012..1927643	30631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 35020.14 Da
• Isoelectric Point: 4.8429

>T92251 WP_001717213.1 NZ_CP025865:1897778-1898695 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYDGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T92251 NZ_CP025865:1897778-1898695 [Escherichia coli]
ATGGATAAAACCTCCCCCTACTACCGCCAGGTCGCTCTGTTGGTGCGAGCATTACCCTGGGTCGCTAACGAGTCCTGTTT
TGCGCTGAAAGGCGGAACGGCGATCAACTTATTCATCCATGATTTTCCCCGATTGTCCGTTGATATCGATCTGGCCTGGA
TACCGCTGGAAAGCAGAGAGGTCGCGCTGCCCAACGTGCGGGAGGCGCTCACCCGTATCGCCGCAGAACTGAAAGCGCAA
ACCGGGATCCGTGCAGTGCTGCAAACCCATAATCCCGATGAGATGCGCATCATTGTCACCACTGACGATGCGCAAATTAA
AATTGAAGTCTCGCCAGTTGCCCGGGGGACACTATATCCTCCGCAAGAACGCGATATCGTCGGAGCCGTGGAGGAAGAAT
TTGGCTTTGCCGCGCTCCCGGTGGTCTCACTGCCCGATTTATACGATGGCAAGCTATGTGCTGCGTTAGATCGCCAGCAC
CCCAGAGATTTTTACGACGTCAAAATGCTCCTTGATACACAAGGAATTGATCGCTCGATATTCAATGGTTTTATCGTGTA
CTTGCTCAGCCATAACCGCCCATTATCCGAAGTGCTTAACCCACGTTGGAAGGATATTACTGAACCCTTTCAAAATGAAT
TCAGCGGTATGACTTTTGAGGAAGTATCACTGGAAGAGCTAAAAGAGGTCCCGGAAAGAATGCTCAGCACGTTGAAAACG
CATTTCACTCAACAGGATTTCGACTTCCTGATGTCATTTAAACGCGGCGAGCCAGACTGGAATTTAGCGCCAGAGCCCCA
CATTCAGAATTTGCCAGCCGTTCAATGGAAGCTGCGCAATATTGGACAGATGTCGGAGGAAAAGCGCGCGCAGTCACTGG
AGAAACTAGAAAAGGTCATGACAGAATGGCTACGTTAG

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT92251 WP_000157137.1 NZ_CP025865:1897012-1897785 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT92251 NZ_CP025865:1897012-1897785 [Escherichia coli]
ATGGCATCAAAATTAAACTGGCTGTTGCAAAACACCTCTCCGGGAAGCCTGGTGTTACAATCGTGGCTGACCCGGAACAA
CATATCACCTTCTCTGGCTTTTAAATACGCGCAAAATGGCTGGTTAATAAAACTGCGGGCTGGCGTTTATGCGCGCGCAG
GACGAGAACCAGACTGGAGTGATGCGCTATGGTGCCTACAAAACCAACTTGAAGCGCCGGTGCATCTGGCCGGACTGAGT
AGTCTCTCCTGGCAGGGCCGTTCCCATTACCTACAACTTAAGCAGAACCAATGCTGGCTTAGCGTTGAAAACAAAGCGAT
ATTGCCAAAATGGTTTAAAGAATTTCCCGCCGTTGAGTGGTTGATGGTATCGGCCCTGAAGCTCCCTGACCTGGATGAAA
AATACCGAACTGAGCTTGAGGTTAAAGGGAAAAGAATTACCGCCAGCACGCCAGAACTGGCTGCCTATGAGCTGTTAAGC
GCAGTCCCTCAGGAAATCTCATTTGAGCACGCTGCCGAGCTTTTTCAGGGACTAGTCAATCTCAACCCACGTAAAGTGGA
GAAGCTGCTTACACTCAGTCAGTCCGTACAAACCAAGCGCCTGTATCTGTTTTTTGCCAGTTTCTATGAACATGCATGGT
TCAAACGCATAGATACCAGCAAAATCAACCTGGGATCGGGCAACCGACAAATCGTTGTTAATGGCAAGCTGAACACGCAA
TACCAGATAACTGTGCCCGAAAATTTTACTAAGAAGGGAATAAGCCATGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2