Detailed information of TA system TA092246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 135105..135743
• Replicon: chromosome
• Accession: NZ_CP025865
• Organism: Escherichia coli strain 504211

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CXX09_RS00625
• Protein ID: WP_000813794.1
• Coordinates: 135567..135743 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CXX09_RS00620
• Protein ID: WP_023485917.1
• Coordinates: 135105..135521 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX09_RS00600	130257..131198	-	942	WP_001251315.1	ABC transporter permease	-
CXX09_RS00605	131199..132212	-	1014	WP_000220416.1	ABC transporter ATP-binding protein	-
CXX09_RS00610	132230..133375	-	1146	WP_001296759.1	ABC transporter substrate-binding protein	-
CXX09_RS00615	133620..135026	-	1407	WP_001296748.1	PLP-dependent aminotransferase family protein	-
CXX09_RS00620	135105..135521	-	417	WP_023485917.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CXX09_RS00625	135567..135743	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CXX09_RS00630	135965..136195	+	231	WP_126133738.1	DUF2554 family protein	-
CXX09_RS00635	136287..138248	-	1962	WP_023485916.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CXX09_RS00640	138321..138857	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
CXX09_RS00645	138910..140124	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	140164..141429	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T92246 WP_000813794.1 NZ_CP025865:c135743-135567 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T92246 NZ_CP025865:c135743-135567 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15236.59 Da
• Isoelectric Point: 4.7386

>AT92246 WP_023485917.1 NZ_CP025865:c135521-135105 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSSLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT92246 NZ_CP025865:c135521-135105 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGTCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB