Detailed information of TA system TA092243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 125938..126473
• Replicon: plasmid p504237_142
• Accession: NZ_CP025863
• Organism: Escherichia coli strain 504237

Toxin (Protein)

• Gene name: relE
• Uniprot ID: T2HS21
• Locus tag: CXX10_RS25670
• Protein ID: WP_000222775.1
• Coordinates: 126186..126473 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T2HSA2
• Locus tag: CXX10_RS25665
• Protein ID: WP_001139207.1
• Coordinates: 125938..126189 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX10_RS25620	121524..121736	+	213	WP_001301409.1	ANR family transcriptional regulator	-
CXX10_RS25625	121982..122443	+	462	WP_001233850.1	thermonuclease family protein	-
CXX10_RS25630	122489..122698	+	210	WP_001353639.1	transcriptional regulator	-
CXX10_RS25635	122736..123326	+	591	WP_000766827.1	DUF2726 domain-containing protein	-
CXX10_RS25640	123566..123814	+	249	WP_000083840.1	replication regulatory protein RepA	-
CXX10_RS25650	124059..124133	+	75	WP_057113837.1	RepA leader peptide Tap	-
CXX10_RS25655	124126..124983	+	858	WP_000130987.1	incFII family plasmid replication initiator RepA	-
CXX10_RS26265	125684..125851	+	168	WP_000841011.1	hypothetical protein	-
CXX10_RS25665	125938..126189	+	252	WP_001139207.1	plasmid stabilization protein	Antitoxin
CXX10_RS25670	126186..126473	+	288	WP_000222775.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXX10_RS26380	126483..127028	+	546	WP_000926983.1	hypothetical protein	-
CXX10_RS25695	127680..128282	-	603	WP_000517689.1	hypothetical protein	-
CXX10_RS26385	128636..128884	+	249	Protein_160	hypothetical protein	-
CXX10_RS25710	128923..130068	-	1146	Protein_161	IS66 family transposase	-
CXX10_RS25720	130122..130799	+	678	WP_001711999.1	IS66 family insertion sequence hypothetical protein	-
CXX10_RS25725	130799..131146	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..142377	142377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11142.17 Da
• Isoelectric Point: 10.6261

>T92243 WP_000222775.1 NZ_CP025863:126186-126473 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Nucleotide

• Length: 288 bp

>T92243 NZ_CP025863:126186-126473 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9216.45 Da
• Isoelectric Point: 4.0927

>AT92243 WP_001139207.1 NZ_CP025863:125938-126189 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT92243 NZ_CP025863:125938-126189 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: T2HS21

Antitoxin

• AlphaFold DB: A0A754EQ65