Detailed information of TA system TA092242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 30181..30706
• Replicon: plasmid p504237_142
• Accession: NZ_CP025863
• Organism: Escherichia coli strain 504237

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: CXX10_RS24980
• Protein ID: WP_001159861.1
• Coordinates: 30401..30706 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: CXX10_RS24975
• Protein ID: WP_000813631.1
• Coordinates: 30181..30399 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX10_RS24940	25857..26975	-	1119	Protein_31	IS3 family transposase	-
CXX10_RS24945	27151..27567	-	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-
CXX10_RS24950	27564..27794	-	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CXX10_RS24960	28286..28903	+	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXX10_RS24965	28937..29626	-	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXX10_RS24975	30181..30399	+	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CXX10_RS24980	30401..30706	+	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
CXX10_RS24985	30707..31513	+	807	WP_126123791.1	site-specific integrase	-
CXX10_RS24990	31870..32250	+	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXX10_RS24995	32247..32594	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CXX10_RS25005	32816..33598	-	783	WP_077822344.1	IS21-like element IS100kyp family helper ATPase IstB	-
CXX10_RS25010	33595..34617	-	1023	WP_001356151.1	IS21 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..142377	142377
	-	inside	IScluster/Tn	-	cssA	25948..56155	30207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T92242 WP_001159861.1 NZ_CP025863:30401-30706 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T92242 NZ_CP025863:30401-30706 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT92242 WP_000813631.1 NZ_CP025863:30181-30399 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92242 NZ_CP025863:30181-30399 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2