Detailed information of TA system TA092241

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 27151..27794
• Replicon: plasmid p504237_142
• Accession: NZ_CP025863
• Organism: Escherichia coli strain 504237

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2VNX7
• Locus tag: CXX10_RS24945
• Protein ID: WP_001044769.1
• Coordinates: 27151..27567 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: T2HRV4
• Locus tag: CXX10_RS24950
• Protein ID: WP_001261281.1
• Coordinates: 27564..27794 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX10_RS24920	22482..23108	+	627	WP_000593827.1	ABC transporter ATP-binding protein	-
CXX10_RS26370	23360..23587	+	228	WP_001731352.1	bacterial lipid A biosynthesis acyltransferase family protein	-
CXX10_RS24930	23981..24130	-	150	WP_001399277.1	ATP-binding protein	-
CXX10_RS24935	24149..25393	-	1245	WP_000115575.1	hypothetical protein	-
CXX10_RS24940	25857..26975	-	1119	Protein_31	IS3 family transposase	-
CXX10_RS24945	27151..27567	-	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	Toxin
CXX10_RS24950	27564..27794	-	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CXX10_RS24960	28286..28903	+	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXX10_RS24965	28937..29626	-	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXX10_RS24975	30181..30399	+	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	-
CXX10_RS24980	30401..30706	+	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	-
CXX10_RS24985	30707..31513	+	807	WP_126123791.1	site-specific integrase	-
CXX10_RS24990	31870..32250	+	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXX10_RS24995	32247..32594	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cssA / csfA	1..142377	142377
	-	inside	IScluster/Tn	-	cssA	25948..56155	30207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15183.63 Da
• Isoelectric Point: 7.7805

>T92241 WP_001044769.1 NZ_CP025863:c27567-27151 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKVKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Nucleotide

• Length: 417 bp

>T92241 NZ_CP025863:c27567-27151 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGGTTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8769.89 Da
• Isoelectric Point: 4.3775

>AT92241 WP_001261281.1 NZ_CP025863:c27794-27564 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPIRPTWGSFLECEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT92241 NZ_CP025863:c27794-27564 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCATCCGGCCAACCTGGGGATCATTCCTAGAGTGCG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A7ZGW5

Antitoxin

• AlphaFold DB: T2HRV4