Detailed information of TA system TA092201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 97397..98022
• Replicon: plasmid p504239_155
• Accession: NZ_CP025861
• Organism: Escherichia coli strain 504239

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CXV92_RS25835
• Protein ID: WP_000911313.1
• Coordinates: 97397..97795 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: CXV92_RS25840
• Protein ID: WP_000450520.1
• Coordinates: 97795..98022 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV92_RS25815	92709..93386	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CXV92_RS25820	93386..93733	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CXV92_RS25825	93753..95324	+	1572	WP_001748001.1	IS66 family transposase	-
CXV92_RS25830	95356..97388	+	2033	Protein_121	type IV conjugative transfer system coupling protein TraD	-
CXV92_RS25835	97397..97795	-	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CXV92_RS25840	97795..98022	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cfaC / cfaC / cfaE / cfaD' / cofA	1..155850	155850

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T92201 WP_000911313.1 NZ_CP025861:c97795-97397 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T92201 NZ_CP025861:c97795-97397 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT92201 WP_000450520.1 NZ_CP025861:c98022-97795 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT92201 NZ_CP025861:c98022-97795 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2