Detailed information of TA system TA092200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 93219..93697
• Replicon: plasmid p504239_101
• Accession: NZ_CP025860
• Organism: Escherichia coli strain 504239

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CXV92_RS25065
• Protein ID: WP_000970001.1
• Coordinates: 93485..93697 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E3PPA5
• Locus tag: CXV92_RS25060
• Protein ID: WP_000079921.1
• Coordinates: 93219..93488 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV92_RS25020	89712..90451	-	740	Protein_94	IS3 family transposase	-
CXV92_RS25025	90489..90620	+	132	WP_064765989.1	type I toxin-antitoxin system Hok family toxin	-
CXV92_RS25030	90904..91164	+	261	WP_000083816.1	replication regulatory protein RepA	-
CXV92_RS25040	91390..91464	+	75	WP_001365705.1	RepA leader peptide Tap	-
CXV92_RS25045	91457..92314	+	858	WP_001379771.1	incFII family plasmid replication initiator RepA	-
CXV92_RS25060	93219..93488	+	270	WP_000079921.1	hypothetical protein	Antitoxin
CXV92_RS25065	93485..93697	+	213	WP_000970001.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV92_RS25075	93878..94081	-	204	Protein_101	IS91 family transposase	-
CXV92_RS25080	94113..94415	+	303	Protein_102	transposase	-
CXV92_RS25085	94537..95750	+	1214	WP_164545847.1	IS3 family transposase	-
CXV92_RS25090	95792..96225	-	434	Protein_104	transposase zinc-binding domain-containing protein	-
CXV92_RS25095	96225..96590	-	366	WP_000124098.1	hypothetical protein	-
CXV92_RS25100	96679..96792	+	114	Protein_106	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaA / cfaB / cfaC / cfaE / cfaE / cfaD' / etpB	1..101617	101617
	-	inside	IScluster/Tn	-	-	74403..96225	21822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8205.40 Da
• Isoelectric Point: 8.5821

>T92200 WP_000970001.1 NZ_CP025860:93485-93697 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMATIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHR

Nucleotide

• Length: 213 bp

>T92200 NZ_CP025860:93485-93697 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCACCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCATCGATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.63 Da
• Isoelectric Point: 9.2950

>AT92200 WP_000079921.1 NZ_CP025860:93219-93488 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNTWFRNQVEAGRQQLKCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT92200 NZ_CP025860:93219-93488 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAAGATGCTTTTATGGCCGCTGCAAAAAACATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTAACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E3PPA5