Detailed information of TA system TA092199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 61507..62066
• Replicon: plasmid p504239_101
• Accession: NZ_CP025860
• Organism: Escherichia coli strain 504239

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: CXV92_RS24850
• Protein ID: WP_000421265.1
• Coordinates: 61507..61782 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: CXV92_RS24855
• Protein ID: WP_001743519.1
• Coordinates: 61782..62066 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV92_RS24820	57323..57718	+	396	WP_000445942.1	plasmid partitioning/stability family protein	-
CXV92_RS24830	59131..59205	+	75	Protein_60	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
CXV92_RS26505	59203..59517	-	315	Protein_61	hypothetical protein	-
CXV92_RS24835	59736..60017	-	282	WP_001105061.1	hypothetical protein	-
CXV92_RS24845	60268..61344	-	1077	WP_000610004.1	hypothetical protein	-
CXV92_RS24850	61507..61782	-	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV92_RS24855	61782..62066	-	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
CXV92_RS24860	62820..64571	-	1752	WP_000038721.1	AAA family ATPase	-
CXV92_RS24865	64903..65100	-	198	WP_001381161.1	hypothetical protein	-
CXV92_RS24870	65313..65879	-	567	WP_000904908.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaA / cfaB / cfaC / cfaE / cfaE / cfaD' / etpB	1..101617	101617
	-	flank	IS/Tn	-	-	65951..69058	3107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T92199 WP_000421265.1 NZ_CP025860:c61782-61507 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T92199 NZ_CP025860:c61782-61507 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT92199 WP_001743519.1 NZ_CP025860:c62066-61782 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT92199 NZ_CP025860:c62066-61782 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1