Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4201852..4202470 Replicon chromosome
Accession NZ_CP025859
Organism Escherichia coli strain 504239

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CXV92_RS20665 Protein ID WP_001291435.1
Coordinates 4201852..4202070 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CXV92_RS20670 Protein ID WP_000344800.1
Coordinates 4202096..4202470 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXV92_RS20630 4197141..4197713 + 573 WP_000779826.1 lipoprotein -
CXV92_RS20635 4197744..4198055 - 312 WP_000409911.1 MGMT family protein -
CXV92_RS20645 4198434..4198787 + 354 WP_000878140.1 DUF1428 domain-containing protein -
CXV92_RS20650 4198829..4200379 - 1551 WP_001484361.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CXV92_RS20655 4200543..4201013 - 471 WP_000136192.1 YlaC family protein -
CXV92_RS20660 4201129..4201680 - 552 WP_000102568.1 maltose O-acetyltransferase -
CXV92_RS20665 4201852..4202070 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
CXV92_RS20670 4202096..4202470 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CXV92_RS20675 4203016..4206165 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
CXV92_RS20680 4206188..4207381 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T92198 WP_001291435.1 NZ_CP025859:c4202070-4201852 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T92198 NZ_CP025859:c4202070-4201852 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT92198 WP_000344800.1 NZ_CP025859:c4202470-4202096 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT92198 NZ_CP025859:c4202470-4202096 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References