Detailed information of TA system TA092197

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3966583..3967262
• Replicon: chromosome
• Accession: NZ_CP025859
• Organism: Escherichia coli strain 504239

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: CXV92_RS19495
• Protein ID: WP_000854680.1
• Coordinates: 3966583..3966924 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: CXV92_RS19500
• Protein ID: WP_000070396.1
• Coordinates: 3966945..3967262 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV92_RS19475	3962662..3963009	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CXV92_RS19480	3963009..3963686	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CXV92_RS19490	3964382..3965488	+	1107	WP_126130422.1	SMEK domain-containing protein	-
CXV92_RS19495	3966583..3966924	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
CXV92_RS19500	3966945..3967262	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
CXV92_RS19505	3967281..3967502	-	222	WP_000691994.1	DUF987 domain-containing protein	-
CXV92_RS19510	3967511..3967987	-	477	WP_000811693.1	RadC family protein	-
CXV92_RS19515	3968003..3968461	-	459	WP_000211838.1	antirestriction protein	-
CXV92_RS19520	3968559..3968798	-	240	WP_000194654.1	DUF905 domain-containing protein	-
CXV92_RS19525	3968875..3969342	-	468	WP_001385283.1	hypothetical protein	-
CXV92_RS19530	3969365..3969808	-	444	WP_000649865.1	hypothetical protein	-
CXV92_RS19535	3969808..3970044	-	237	WP_001144031.1	hypothetical protein	-
CXV92_RS19540	3970085..3970786	-	702	WP_000189411.1	WYL domain-containing protein	-
CXV92_RS19545	3971003..3971824	-	822	WP_001385280.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vgrG/tssI / gmhA/lpcA	3921461..4018755	97294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T92197 WP_000854680.1 NZ_CP025859:c3966924-3966583 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T92197 NZ_CP025859:c3966924-3966583 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT92197 WP_000070396.1 NZ_CP025859:c3967262-3966945 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT92197 NZ_CP025859:c3967262-3966945 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7