Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1872424..1873078 Replicon chromosome
Accession NZ_CP025859
Organism Escherichia coli strain 504239

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag CXV92_RS09285 Protein ID WP_000244781.1
Coordinates 1872424..1872831 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXV92_RS09290 Protein ID WP_000354046.1
Coordinates 1872812..1873078 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXV92_RS09265 1868381..1870114 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
CXV92_RS09270 1870120..1870830 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXV92_RS09275 1870855..1871751 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CXV92_RS09280 1871863..1872384 + 522 WP_001055874.1 flavodoxin FldB -
CXV92_RS09285 1872424..1872831 - 408 WP_000244781.1 protein YgfX Toxin
CXV92_RS09290 1872812..1873078 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXV92_RS09295 1873321..1874301 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
CXV92_RS09300 1874497..1875156 - 660 WP_000250274.1 hemolysin III family protein -
CXV92_RS09305 1875320..1875631 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CXV92_RS09310 1875676..1877109 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
CXV92_RS09315 1877166..1877909 - 744 WP_000951939.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T92191 WP_000244781.1 NZ_CP025859:c1872831-1872424 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T92191 NZ_CP025859:c1872831-1872424 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT92191 WP_000354046.1 NZ_CP025859:c1873078-1872812 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT92191 NZ_CP025859:c1873078-1872812 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References