Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 423693..424219 Replicon chromosome
Accession NZ_CP025859
Organism Escherichia coli strain 504239

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CXV92_RS02185 Protein ID WP_000323025.1
Coordinates 423693..423980 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CXV92_RS02190 Protein ID WP_000534858.1
Coordinates 423980..424219 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXV92_RS02140 418717..418932 - 216 WP_000839590.1 phage lysis protein EssD -
CXV92_RS26570 419152..419322 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
CXV92_RS02145 419686..419901 - 216 WP_000066484.1 cold shock-like protein CspB -
CXV92_RS02150 420202..420414 + 213 WP_000087756.1 cold shock-like protein CspF -
CXV92_RS02155 420469..420558 + 90 WP_120795389.1 hypothetical protein -
CXV92_RS02160 420836..421588 - 753 WP_001047135.1 antitermination protein -
CXV92_RS02165 421602..422651 - 1050 WP_001265200.1 DUF968 domain-containing protein -
CXV92_RS02170 422653..422931 - 279 WP_012304870.1 hypothetical protein -
CXV92_RS02175 422998..423249 - 252 WP_000980994.1 hypothetical protein -
CXV92_RS02180 423466..423621 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CXV92_RS02185 423693..423980 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CXV92_RS02190 423980..424219 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CXV92_RS02195 424244..424453 + 210 Protein_424 hypothetical protein -
CXV92_RS02200 424452..424580 - 129 Protein_425 dicB transcriptional regulator DicC -
CXV92_RS02205 424664..425071 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
CXV92_RS02210 425238..425393 + 156 WP_000379575.1 DUF1391 family protein -
CXV92_RS02215 425395..425970 + 576 WP_000344953.1 hypothetical protein -
CXV92_RS02220 426457..426645 + 189 WP_000854559.1 cell division inhibition protein DicB -
CXV92_RS02225 426642..426833 + 192 WP_001083278.1 lysis protein YdfD -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 394585..444053 49468


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T92179 WP_000323025.1 NZ_CP025859:c423980-423693 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T92179 NZ_CP025859:c423980-423693 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT92179 WP_000534858.1 NZ_CP025859:c424219-423980 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT92179 NZ_CP025859:c424219-423980 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References