Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 274155..274793 Replicon chromosome
Accession NZ_CP025859
Organism Escherichia coli strain 504239

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CXV92_RS01445 Protein ID WP_000813794.1
Coordinates 274155..274331 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CXV92_RS01450 Protein ID WP_001270286.1
Coordinates 274377..274793 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXV92_RS01425 269774..270988 - 1215 WP_001324881.1 BenE family transporter YdcO -
CXV92_RS01430 271041..271577 + 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
CXV92_RS01435 271650..273611 + 1962 WP_001295654.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CXV92_RS01440 273703..273933 - 231 WP_000494244.1 YncJ family protein -
CXV92_RS01445 274155..274331 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CXV92_RS01450 274377..274793 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CXV92_RS01455 274872..276278 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
CXV92_RS01460 276523..277668 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
CXV92_RS01465 277686..278699 + 1014 WP_000220382.1 ABC transporter ATP-binding protein -
CXV92_RS01470 278700..279641 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T92178 WP_000813794.1 NZ_CP025859:274155-274331 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T92178 NZ_CP025859:274155-274331 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT92178 WP_001270286.1 NZ_CP025859:274377-274793 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT92178 NZ_CP025859:274377-274793 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References