Detailed information of TA system TA092177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 42268..42827
• Replicon: plasmid p504838_88
• Accession: NZ_CP025858
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: relE
• Uniprot ID: E3PPG0
• Locus tag: CXV93_RS26430
• Protein ID: WP_000421265.1
• Coordinates: 42552..42827 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: E3PPG1
• Locus tag: CXV93_RS26425
• Protein ID: WP_001743519.1
• Coordinates: 42268..42552 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS26405	37680..38246	+	567	WP_000904908.1	recombinase family protein	-
CXV93_RS26410	38459..38656	+	198	WP_001381161.1	hypothetical protein	-
CXV93_RS26415	39105..39802	+	698	Protein_41	IS1 family transposase	-
CXV93_RS26420	39795..41514	+	1720	Protein_42	AAA family ATPase	-
CXV93_RS26425	42268..42552	+	285	WP_001743519.1	ribbon-helix-helix domain-containing protein	Antitoxin
CXV93_RS26430	42552..42827	+	276	WP_000421265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV93_RS26435	42990..44066	+	1077	WP_000610005.1	hypothetical protein	-
CXV93_RS26445	44418..44885	-	468	Protein_46	IS3 family transposase	-
CXV93_RS26450	44966..46474	-	1509	WP_126125604.1	group II intron reverse transcriptase/maturase	-
CXV93_RS26460	47147..47572	-	426	Protein_48	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaE / cfaC / cfaB / cfaA	1..88871	88871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10742.45 Da
• Isoelectric Point: 9.9324

>T92177 WP_000421265.1 NZ_CP025858:42552-42827 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQASVILLTHPRMGEQLFQFELREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T92177 NZ_CP025858:42552-42827 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCATCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACTCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGTCTGTGGCATACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10490.95 Da
• Isoelectric Point: 5.6546

>AT92177 WP_001743519.1 NZ_CP025858:42268-42552 [Escherichia coli]
MQMKNNCAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQIIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT92177 NZ_CP025858:42268-42552 [Escherichia coli]
ATGCAAATGAAAAACAATTGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGGTTATCAAGCAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTAACATCCGGACAGATTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: E3PPG0

Antitoxin

• AlphaFold DB: E3PPG1