Detailed information of TA system TA092176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1763..2241
• Replicon: plasmid p504838_88
• Accession: NZ_CP025858
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: CXV93_RS26205
• Protein ID: WP_000969994.1
• Coordinates: 2029..2241 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E3PPA5
• Locus tag: CXV93_RS26200
• Protein ID: WP_000079921.1
• Coordinates: 1763..2032 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS26190	1..858	+	858	WP_000130956.1	incFII family plasmid replication initiator RepA	-
CXV93_RS26200	1763..2032	+	270	WP_000079921.1	hypothetical protein	Antitoxin
CXV93_RS26205	2029..2241	+	213	WP_000969994.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXV93_RS26210	2477..3685	-	1209	WP_001695118.1	IS91 family transposase	-
CXV93_RS26215	3666..3938	-	273	WP_001695087.1	hypothetical protein	-
CXV93_RS27060	3932..4021	+	90	Protein_6	invasion protein	-
CXV93_RS27065	4016..4246	+	231	Protein_7	transposase	-
CXV93_RS26225	4368..5581	+	1214	WP_164544955.1	IS3 family transposase	-
CXV93_RS26230	5752..6152	+	401	Protein_9	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	cfaD' / cfaE / cfaE / cfaC / cfaB / cfaA	1..88871	88871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-66)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8175.38 Da
• Isoelectric Point: 8.5821

>T92176 WP_000969994.1 NZ_CP025858:2029-2241 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVHR

Nucleotide

• Length: 213 bp

>T92176 NZ_CP025858:2029-2241 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCATCGATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10224.63 Da
• Isoelectric Point: 9.2950

>AT92176 WP_000079921.1 NZ_CP025858:1763-2032 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKNMDRNGSQLIRDFMRQTVERQHNTWFRNQVEAGRQQLKCGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT92176 NZ_CP025858:1763-2032 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAAGATGCTTTTATGGCCGCTGCAAAAAACATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGGCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTAACCAGGTTG
AGGCAGGACGTCAGCAACTCAAGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E3PPA5