Detailed information of TA system TA092162

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4992527..4993359
• Replicon: chromosome
• Accession: NZ_CP025856
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CXV93_RS24730
• Protein ID: WP_000854762.1
• Coordinates: 4992527..4992901 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CXV93_RS24735
• Protein ID: WP_001295723.1
• Coordinates: 4992991..4993359 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS24700	4988478..4989217	-	740	Protein_4742	IS3 family transposase	-
CXV93_RS24705	4989267..4990442	+	1176	Protein_4743	IS66-like element ISEc23 family transposase	-
CXV93_RS27040	4990434..4990585	+	152	Protein_4744	restriction endonuclease subunit S	-
CXV93_RS24710	4990695..4991828	-	1134	WP_126125585.1	IS110 family transposase	-
CXV93_RS24715	4991889..4992077	+	189	Protein_4746	IS66 family transposase	-
CXV93_RS24720	4992198..4992311	-	114	WP_126125592.1	DUF957 domain-containing protein	-
CXV93_RS24725	4992324..4992530	-	207	WP_000976829.1	hypothetical protein	-
CXV93_RS24730	4992527..4992901	-	375	WP_000854762.1	TA system toxin CbtA family protein	Toxin
CXV93_RS24735	4992991..4993359	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXV93_RS24740	4993522..4993743	-	222	WP_000692341.1	DUF987 domain-containing protein	-
CXV93_RS24745	4993806..4994282	-	477	WP_001186774.1	RadC family protein	-
CXV93_RS24750	4994298..4994771	-	474	WP_000855059.1	antirestriction protein	-
CXV93_RS24760	4995113..4995931	-	819	WP_126125586.1	DUF945 domain-containing protein	-
CXV93_RS24770	4996086..4996244	-	159	WP_001433433.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4962442..5028294	65852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14094.15 Da
• Isoelectric Point: 7.1881

>T92162 WP_000854762.1 NZ_CP025856:c4992901-4992527 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T92162 NZ_CP025856:c4992901-4992527 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT92162 WP_001295723.1 NZ_CP025856:c4993359-4992991 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT92162 NZ_CP025856:c4993359-4992991 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAATCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52