Detailed information of TA system TA092160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4433548..4434173
• Replicon: chromosome
• Accession: NZ_CP025856
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U9YZ02
• Locus tag: CXV93_RS22055
• Protein ID: WP_000911329.1
• Coordinates: 4433775..4434173 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CXV93_RS22050
• Protein ID: WP_001696518.1
• Coordinates: 4433548..4433775 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS22025	4429350..4429820	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CXV93_RS22030	4429820..4430392	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CXV93_RS22035	4430538..4431416	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CXV93_RS22040	4431433..4432467	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CXV93_RS22045	4432680..4433393	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CXV93_RS22050	4433548..4433775	+	228	WP_001696518.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CXV93_RS22055	4433775..4434173	+	399	WP_000911329.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CXV93_RS22060	4434320..4435183	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CXV93_RS22065	4435198..4437213	+	2016	WP_001388162.1	tRNA cytosine(34) acetyltransferase TmcA	-
CXV93_RS22070	4437287..4437985	+	699	WP_126125567.1	esterase	-
CXV93_RS22075	4438066..4438266	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4287428..4494693	207265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.23 Da
• Isoelectric Point: 8.5325

>T92160 WP_000911329.1 NZ_CP025856:4433775-4434173 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T92160 NZ_CP025856:4433775-4434173 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.66 Da
• Isoelectric Point: 4.3867

>AT92160 WP_001696518.1 NZ_CP025856:4433548-4433775 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIVIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT92160 NZ_CP025856:4433548-4433775 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGTGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XW84