Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2464839..2465336 Replicon chromosome
Accession NZ_CP025856
Organism Escherichia coli strain 504838

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXV93_RS12250 Protein ID WP_001173343.1
Coordinates 2464839..2465012 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXV93_RS12255 Protein ID WP_000398619.1
Coordinates 2465040..2465336 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXV93_RS12235 2460524..2461450 + 927 Protein_2360 lysine decarboxylase CadA -
CXV93_RS12240 2461509..2462966 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
CXV93_RS12245 2463203..2464720 + 1518 WP_001295074.1 lysine--tRNA ligase -
CXV93_RS12250 2464839..2465012 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXV93_RS12255 2465040..2465336 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXV93_RS12260 2465563..2465835 - 273 WP_000405647.1 N-acetyltransferase -
CXV93_RS12265 2465847..2466077 - 231 WP_000371698.1 (4Fe-4S)-binding protein -
CXV93_RS12270 2466258..2467889 + 1632 WP_001216486.1 sensor histidine kinase -
CXV93_RS12275 2467886..2468605 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92154 WP_001173343.1 NZ_CP025856:c2465012-2464839 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92154 NZ_CP025856:c2465012-2464839 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92154 WP_000398619.1 NZ_CP025856:c2465336-2465040 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92154 NZ_CP025856:c2465336-2465040 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References