Detailed information of TA system TA092132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4192463..4193168
• Replicon: chromosome
• Accession: NZ_CP025851
• Organism: Escherichia coli strain 510016

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CXX11_RS20830
• Protein ID: WP_000539521.1
• Coordinates: 4192782..4193168 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CXX11_RS20825
• Protein ID: WP_001280945.1
• Coordinates: 4192463..4192792 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX11_RS20800	4187907..4188818	+	912	WP_001400150.1	glutathione ABC transporter permease GsiD	-
CXX11_RS20805	4188996..4189688	+	693	Protein_3973	CHASE9 sensor domain-containing protein	-
CXX11_RS20810	4189745..4190442	+	698	Protein_3974	IS1 family transposase	-
CXX11_RS20815	4190452..4191057	+	606	WP_071597936.1	EAL domain-containing protein	-
CXX11_RS20820	4191065..4192393	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
CXX11_RS20825	4192463..4192792	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CXX11_RS20830	4192782..4193168	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXX11_RS20835	4193394..4194719	-	1326	WP_001404917.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CXX11_RS20840	4194932..4195315	+	384	WP_000497137.1	biofilm formation regulator BssR	-
CXX11_RS20845	4195426..4196541	+	1116	WP_000554946.1	aldose sugar dehydrogenase YliI	-
CXX11_RS20850	4196538..4197164	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4189939..4190442	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T92132 WP_000539521.1 NZ_CP025851:c4193168-4192782 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T92132 NZ_CP025851:c4193168-4192782 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT92132 WP_001280945.1 NZ_CP025851:c4192792-4192463 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT92132 NZ_CP025851:c4192792-4192463 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6