Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2981747..2982244 Replicon chromosome
Accession NZ_CP025851
Organism Escherichia coli strain 510016

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CXX11_RS14735 Protein ID WP_001173343.1
Coordinates 2982071..2982244 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CXX11_RS14730 Protein ID WP_000398619.1
Coordinates 2981747..2982043 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX11_RS14710 2978478..2979197 - 720 WP_000611288.1 two-component system response regulator DcuR -
CXX11_RS14715 2979194..2980825 - 1632 WP_001406504.1 sensor histidine kinase -
CXX11_RS14720 2981006..2981236 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
CXX11_RS14725 2981248..2981520 + 273 WP_000405651.1 N-acetyltransferase -
CXX11_RS14730 2981747..2982043 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CXX11_RS14735 2982071..2982244 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CXX11_RS14740 2982363..2983571 - 1209 Protein_2818 lysine--tRNA ligase -
CXX11_RS14745 2983589..2984286 - 698 Protein_2819 IS1 family transposase -
CXX11_RS14750 2984277..2984657 - 381 Protein_2820 hypothetical protein -
CXX11_RS14755 2984894..2986351 - 1458 WP_074507863.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2935923..2998137 62214
- flank IS/Tn - - 2983589..2984092 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T92128 WP_001173343.1 NZ_CP025851:2982071-2982244 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T92128 NZ_CP025851:2982071-2982244 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT92128 WP_000398619.1 NZ_CP025851:2981747-2982043 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT92128 NZ_CP025851:2981747-2982043 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References