Detailed information of TA system TA092112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 58895..59420
• Replicon: plasmid p600468_158
• Accession: NZ_CP025850
• Organism: Escherichia coli strain 600468

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CXX14_RS23725
• Protein ID: WP_001513771.1
• Coordinates: 58895..59200 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: B1EFQ7
• Locus tag: CXX14_RS23730
• Protein ID: WP_000813636.1
• Coordinates: 59202..59420 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX14_RS24605	54921..55295	-	375	WP_032195917.1	hypothetical protein	-
CXX14_RS23710	55295..55927	-	633	WP_000312330.1	ParA family protein	-
CXX14_RS23715	56775..57530	-	756	WP_001513769.1	replication initiation protein RepE	-
CXX14_RS23720	58088..58894	-	807	WP_024203647.1	site-specific integrase	-
CXX14_RS23725	58895..59200	-	306	WP_001513771.1	type II toxin-antitoxin system toxin CcdB	Toxin
CXX14_RS23730	59202..59420	-	219	WP_000813636.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CXX14_RS23750	59943..60200	+	258	WP_000343092.1	hypothetical protein	-
CXX14_RS23755	60200..60742	+	543	WP_024203648.1	hypothetical protein	-
CXX14_RS23760	60986..61828	+	843	WP_164717674.1	polysaccharide deacetylase family protein	-
CXX14_RS23765	61831..62919	+	1089	WP_064717534.1	putative hexosyltransferase CapU	-
CXX14_RS23770	62924..63874	+	951	WP_001420716.1	virulence factor VirK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	etpB / csaE	1..158237	158237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11649.46 Da
• Isoelectric Point: 5.6919

>T92112 WP_001513771.1 NZ_CP025850:c59200-58895 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSLELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T92112 NZ_CP025850:c59200-58895 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCTTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8400.40 Da
• Isoelectric Point: 5.0807

>AT92112 WP_000813636.1 NZ_CP025850:c59420-59202 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENREGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92112 NZ_CP025850:c59420-59202 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCGGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: B1EFQ7