Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2789563..2790188 Replicon chromosome
Accession NZ_CP025849
Organism Escherichia coli strain 600468

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CXX14_RS13400 Protein ID WP_000911329.1
Coordinates 2789790..2790188 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CXX14_RS13395 Protein ID WP_000450524.1
Coordinates 2789563..2789790 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX14_RS13370 2785365..2785835 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CXX14_RS13375 2785835..2786407 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CXX14_RS13380 2786553..2787431 + 879 WP_001311023.1 4-hydroxy-tetrahydrodipicolinate synthase -
CXX14_RS13385 2787448..2788482 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CXX14_RS13390 2788695..2789408 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CXX14_RS13395 2789563..2789790 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CXX14_RS13400 2789790..2790188 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CXX14_RS13405 2790335..2791198 + 864 WP_001267498.1 neutral zinc metallopeptidase -
CXX14_RS13410 2791213..2793228 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
CXX14_RS13415 2793302..2794000 + 699 WP_000679823.1 esterase -
CXX14_RS13420 2794110..2794310 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T92100 WP_000911329.1 NZ_CP025849:2789790-2790188 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T92100 NZ_CP025849:2789790-2790188 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT92100 WP_000450524.1 NZ_CP025849:2789563-2789790 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT92100 NZ_CP025849:2789563-2789790 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References