Detailed information of TA system TA092043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2396999..2397575
• Replicon: chromosome
• Accession: NZ_CP025842
• Organism: Escherichia coli strain 720632

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: CXX37_RS12250
• Protein ID: WP_001297643.1
• Coordinates: 2397288..2397575 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: CXX37_RS12245
• Protein ID: WP_061358923.1
• Coordinates: 2396999..2397301 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX37_RS12230	2393595..2395745	+	2151	WP_001295524.1	pyruvate/proton symporter BtsT	-
CXX37_RS12235	2395795..2395998	+	204	WP_000467859.1	YbdD/YjiX family protein	-
CXX37_RS12240	2396009..2396965	+	957	WP_001297640.1	GTPase	-
CXX37_RS12245	2396999..2397301	-	303	WP_061358923.1	BrnA antitoxin family protein	Antitoxin
CXX37_RS12250	2397288..2397575	-	288	WP_001297643.1	BrnT family toxin	Toxin
CXX37_RS12255	2397774..2398470	-	697	Protein_2356	IS1 family transposase	-
CXX37_RS12260	2398525..2398692	-	168	Protein_2357	YbfB/YjiJ family MFS transporter	-
CXX37_RS12265	2398760..2399620	-	861	WP_001317601.1	YjiK family protein	-
CXX37_RS12270	2399685..2399942	-	258	WP_000666413.1	DUF3343 domain-containing protein	-
CXX37_RS12275	2399939..2400706	-	768	WP_001297641.1	hypothetical protein	-
CXX37_RS12280	2400716..2401867	-	1152	WP_001330164.1	2-hydroxyacyl-CoA dehydratase subunit D	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2397774..2397983	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T92043 WP_001297643.1 NZ_CP025842:c2397575-2397288 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T92043 NZ_CP025842:c2397575-2397288 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11550.04 Da
• Isoelectric Point: 9.4646

>AT92043 WP_061358923.1 NZ_CP025842:c2397301-2396999 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNVERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT92043 NZ_CP025842:c2397301-2396999 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGTCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9