Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2756096..2756750 Replicon chromosome
Accession NZ_CP025840
Organism Escherichia coli strain 214-4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CXX41_RS13630 Protein ID WP_000244777.1
Coordinates 2756343..2756750 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXX41_RS13625 Protein ID WP_000354046.1
Coordinates 2756096..2756362 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX41_RS13600 2751265..2752008 + 744 WP_000951948.1 SDR family oxidoreductase -
CXX41_RS13605 2752065..2753498 - 1434 WP_157916743.1 6-phospho-beta-glucosidase BglA -
CXX41_RS13610 2753543..2753854 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
CXX41_RS13615 2754018..2754677 + 660 WP_129547209.1 hemolysin III family protein -
CXX41_RS13620 2754873..2755853 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CXX41_RS13625 2756096..2756362 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXX41_RS13630 2756343..2756750 + 408 WP_000244777.1 toxin CptA Toxin
CXX41_RS13635 2756790..2757311 - 522 WP_001055874.1 flavodoxin FldB -
CXX41_RS13640 2757423..2758319 + 897 WP_097344882.1 site-specific tyrosine recombinase XerD -
CXX41_RS13645 2758344..2759054 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXX41_RS13650 2759060..2760793 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T92012 WP_000244777.1 NZ_CP025840:2756343-2756750 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T92012 NZ_CP025840:2756343-2756750 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT92012 WP_000354046.1 NZ_CP025840:2756096-2756362 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT92012 NZ_CP025840:2756096-2756362 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References