Detailed information of TA system TA092011

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2622553..2623388
• Replicon: chromosome
• Accession: NZ_CP025840
• Organism: Escherichia coli strain 214-4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: CXX41_RS12880
• Protein ID: WP_000854821.1
• Coordinates: 2622553..2622930 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: CXX41_RS12885
• Protein ID: WP_001285610.1
• Coordinates: 2623020..2623388 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX41_RS12845	2617634..2618233	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
CXX41_RS12850	2618236..2619213	+	978	WP_129547197.1	type II secretion system minor pseudopilin GspK	-
CXX41_RS12855	2619210..2620388	+	1179	WP_000094962.1	type II secretion system protein GspL	-
CXX41_RS12860	2620390..2620926	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
CXX41_RS12865	2621207..2622049	-	843	WP_129547198.1	DUF4942 domain-containing protein	-
CXX41_RS12870	2622134..2622331	-	198	WP_085949090.1	DUF957 domain-containing protein	-
CXX41_RS12875	2622359..2622556	-	198	Protein_2475	hypothetical protein	-
CXX41_RS12880	2622553..2622930	-	378	WP_000854821.1	type IV toxin-antitoxin system toxin CbtA	Toxin
CXX41_RS12885	2623020..2623388	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CXX41_RS12890	2623468..2623689	-	222	WP_000692350.1	DUF987 domain-containing protein	-
CXX41_RS12895	2623776..2624252	-	477	WP_001384108.1	RadC family protein	-
CXX41_RS12900	2624268..2624750	-	483	WP_000206657.1	antirestriction protein	-
CXX41_RS12905	2624842..2625660	-	819	WP_001175136.1	DUF945 domain-containing protein	-
CXX41_RS12910	2625750..2625983	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T92011 WP_000854821.1 NZ_CP025840:c2622930-2622553 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T92011 NZ_CP025840:c2622930-2622553 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT92011 WP_001285610.1 NZ_CP025840:c2623388-2623020 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT92011 NZ_CP025840:c2623388-2623020 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54