Detailed information of TA system TA092003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 54221..54926
• Replicon: chromosome
• Accession: NZ_CP025840
• Organism: Escherichia coli strain 214-4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: CXX41_RS00360
• Protein ID: WP_000539521.1
• Coordinates: 54221..54607 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CXX41_RS00365
• Protein ID: WP_001280945.1
• Coordinates: 54597..54926 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX41_RS00340	50225..50851	+	627	WP_097560969.1	glutathione S-transferase GstB	-
CXX41_RS00345	50848..51963	-	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
CXX41_RS00350	52074..52457	-	384	WP_000497137.1	biofilm formation regulator BssR	-
CXX41_RS00355	52670..53995	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
CXX41_RS00360	54221..54607	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CXX41_RS00365	54597..54926	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
CXX41_RS00370	54996..56324	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
CXX41_RS00375	56332..58680	-	2349	WP_129546977.1	EAL domain-containing protein	-
CXX41_RS00380	58858..59769	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T92003 WP_000539521.1 NZ_CP025840:54221-54607 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T92003 NZ_CP025840:54221-54607 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT92003 WP_001280945.1 NZ_CP025840:54597-54926 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT92003 NZ_CP025840:54597-54926 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6