Detailed information of TA system TA091998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 960778..961337
• Replicon: chromosome
• Accession: NZ_CP025819
• Organism: Brucella melitensis strain CIT21

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A7U8PD14
• Locus tag: C1H22_RS05035
• Protein ID: WP_002966807.1
• Coordinates: 961092..961337 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: C0RIV1
• Locus tag: C1H22_RS05030
• Protein ID: WP_004683740.1
• Coordinates: 960778..961092 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1H22_RS04980	956483..956686	-	204	WP_004683737.1	hypothetical protein	-
C1H22_RS04985	956689..956904	-	216	WP_005968947.1	hypothetical protein	-
C1H22_RS04990	956901..957104	-	204	WP_002964088.1	hypothetical protein	-
C1H22_RS04995	957154..957858	+	705	WP_005968944.1	hypothetical protein	-
C1H22_RS05000	957855..958085	+	231	WP_002964091.1	hypothetical protein	-
C1H22_RS05005	958082..958357	+	276	WP_002964092.1	hypothetical protein	-
C1H22_RS05010	958380..958856	+	477	WP_002966806.1	hypothetical protein	-
C1H22_RS05015	959202..959912	+	711	WP_118874251.1	porin family protein	-
C1H22_RS05020	960260..960430	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
C1H22_RS17035	960429..960662	+	234	WP_002969999.1	hypothetical protein	-
C1H22_RS05030	960778..961092	-	315	WP_004683740.1	BrnA antitoxin family protein	Antitoxin
C1H22_RS05035	961092..961337	-	246	WP_002966807.1	BrnT family toxin	Toxin
C1H22_RS05040	961470..962108	+	639	Protein_925	tRNA-guanine transglycosylase	-
C1H22_RS05045	962178..962935	+	758	WP_080723468.1	IS5-like element IS711 family transposase	-
C1H22_RS05050	962936..963628	+	693	WP_006135935.1	hypothetical protein	-
C1H22_RS05055	964040..965491	+	1452	WP_006135932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	954711..968807	14096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9162.82 Da
• Isoelectric Point: 10.3158

>T91998 WP_002966807.1 NZ_CP025819:c961337-961092 [Brucella melitensis]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRSASQKERK
L

Nucleotide

• Length: 246 bp

>T91998 NZ_CP025819:c961337-961092 [Brucella melitensis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTTCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11653.48 Da
• Isoelectric Point: 10.5824

>AT91998 WP_004683740.1 NZ_CP025819:c961092-960778 [Brucella melitensis]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDKQLAKAKPFKESFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT91998 NZ_CP025819:c961092-960778 [Brucella melitensis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAGGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATAAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAATCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U8PD14

Antitoxin

• AlphaFold DB: C0RIV1