Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1218470..1219095 Replicon chromosome
Accession NZ_CP025799
Organism Dickeya zeae strain MS2

Toxin (Protein)


Gene name Hha Uniprot ID E0SD87
Locus tag C1O30_RS05590 Protein ID WP_009114000.1
Coordinates 1218470..1218673 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID D2BUK3
Locus tag C1O30_RS05595 Protein ID WP_012883774.1
Coordinates 1218727..1219095 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1O30_RS05560 1214097..1214435 + 339 WP_002208627.1 P-II family nitrogen regulator -
C1O30_RS05565 1214479..1215771 + 1293 WP_102801261.1 ammonium transporter AmtB -
C1O30_RS05570 1215892..1216755 - 864 WP_023639171.1 acyl-CoA thioesterase II -
C1O30_RS05575 1216973..1217554 + 582 WP_102801262.1 YbaY family lipoprotein -
C1O30_RS05580 1217595..1217921 - 327 WP_012883773.1 MGMT family protein -
C1O30_RS05590 1218470..1218673 - 204 WP_009114000.1 hemolysin expression modulator Hha Toxin
C1O30_RS05595 1218727..1219095 - 369 WP_012883774.1 Hha toxicity modulator TomB Antitoxin
C1O30_RS05600 1219605..1219748 - 144 WP_012770724.1 type B 50S ribosomal protein L36 -
C1O30_RS05605 1219764..1220015 - 252 WP_019844635.1 type B 50S ribosomal protein L31 -
C1O30_RS05610 1220191..1223337 - 3147 WP_019844634.1 multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8133.51 Da        Isoelectric Point: 8.8500

>T91970 WP_009114000.1 NZ_CP025799:c1218673-1218470 [Dickeya zeae]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Download         Length: 204 bp

>T91970 NZ_CP025799:c1218673-1218470 [Dickeya zeae]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGTACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCTAACGACGAACTTGAAATGTTCTATTCAGCTGCAGACCATCGTCTGGCTGAGCTAACGATGAATAAAC
TTTACGACAAAGTGCCAACTGCCGTGTGGAAATACGTTCGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14186.04 Da        Isoelectric Point: 5.1811

>AT91970 WP_012883774.1 NZ_CP025799:c1219095-1218727 [Dickeya zeae]
MDEYTPQHYDIAQLRFLCENLHDESIATLGDSSRGWVNDPTSAVNLQLNELIEHIAAFVVTYKIKYPHEAALCERVEKYL
DDTYILFSNYGINDSELQKWKKSKSQLFRMFSEKSVCTVVKT

Download         Length: 369 bp

>AT91970 NZ_CP025799:c1219095-1218727 [Dickeya zeae]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
AACATTAGGTGACAGCAGTCGTGGCTGGGTGAATGATCCGACCTCTGCGGTCAATCTTCAACTAAACGAACTTATCGAAC
ACATTGCGGCATTTGTTGTTACCTACAAAATAAAATATCCGCATGAAGCGGCGCTGTGTGAGCGGGTTGAAAAATACCTG
GACGATACTTATATTCTTTTCAGCAACTACGGTATAAATGATTCAGAGTTGCAGAAATGGAAAAAATCCAAGTCGCAATT
ATTCAGAATGTTCTCAGAAAAGAGCGTCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X3RNQ0

Antitoxin

Source ID Structure
AlphaFold DB A0A2K9QCC2

References