Detailed information of TA system TA091958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 6809..7388
• Replicon: plasmid pl3
• Accession: NZ_CP025784
• Organism: Iodobacter fluviatilis strain PCH194

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: C1H71_RS20465
• Protein ID: WP_130108428.1
• Coordinates: 6809..7093 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: C1H71_RS20470
• Protein ID: WP_130108429.1
• Coordinates: 7080..7388 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1H71_RS20440	2845..4098	-	1254	WP_130108431.1	PAAR domain-containing protein	-
C1H71_RS20445	4123..4452	-	330	WP_130108432.1	mobilization protein	-
C1H71_RS20450	4644..5762	+	1119	WP_130108433.1	MobA/MobL family protein	-
C1H71_RS20455	5828..6064	+	237	WP_130108434.1	hypothetical protein	-
C1H71_RS20460	6067..6777	+	711	WP_130108435.1	hypothetical protein	-
C1H71_RS20465	6809..7093	+	285	WP_130108428.1	BrnT family toxin	Toxin
C1H71_RS20470	7080..7388	+	309	WP_130108429.1	BrnA antitoxin family protein	Antitoxin
C1H71_RS20475	7524..8483	+	960	WP_130108430.1	replication initiation protein	-
C1H71_RS20480	9144..10397	-	1254	WP_130108431.1	PAAR domain-containing protein	-
C1H71_RS20485	10422..10751	-	330	WP_130108432.1	mobilization protein	-
C1H71_RS20490	10943..12061	+	1119	WP_130108433.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..12328	12328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-87)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10853.23 Da
• Isoelectric Point: 5.6017

>T91958 WP_130108428.1 NZ_CP025784:6809-7093 [Iodobacter fluviatilis]
MSRVFEWDEAKAASNFRKHGVHFEEAAFVFDDPLAVSIQDRIENGEARWQTIGMAGGCLLLLVAHTVRFEDVEVVEIISA
RRTDKKERYRYEHG

Nucleotide

• Length: 285 bp

>T91958 NZ_CP025784:6809-7093 [Iodobacter fluviatilis]
ATGAGTAGAGTTTTTGAGTGGGACGAGGCTAAAGCGGCGAGTAATTTTCGCAAGCATGGGGTTCATTTCGAGGAGGCGGC
ATTCGTGTTTGACGACCCCTTGGCGGTTTCGATTCAAGACCGAATAGAAAACGGCGAGGCGCGATGGCAAACCATCGGCA
TGGCCGGAGGGTGTTTGCTGCTATTGGTGGCGCATACGGTGCGCTTTGAGGATGTGGAAGTCGTTGAAATCATTTCCGCA
CGACGTACCGACAAAAAGGAGAGATACCGCTATGAGCATGGTTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11469.16 Da
• Isoelectric Point: 9.5113

>AT91958 WP_130108429.1 NZ_CP025784:7080-7388 [Iodobacter fluviatilis]
MSMVRHEQGKLPALTAQRRAELEALAKMPDASIDYSDIPPLTDAFWQNAVSNPFYKPTKTHATVRLDSDVMAWLKSQGKG
YQTRLNAILRKAMLEDIDKAHG

Nucleotide

• Length: 309 bp

>AT91958 NZ_CP025784:7080-7388 [Iodobacter fluviatilis]
ATGAGCATGGTTAGACACGAGCAAGGGAAGCTACCGGCCTTAACGGCCCAACGTCGCGCAGAGCTTGAAGCACTAGCGAA
AATGCCGGATGCGAGCATCGACTACAGCGACATTCCGCCGTTGACTGATGCGTTTTGGCAAAACGCCGTGAGCAATCCCT
TTTACAAGCCGACCAAGACGCACGCGACTGTGCGGCTGGACTCGGATGTGATGGCTTGGCTGAAATCGCAGGGAAAGGGC
TATCAGACGCGGCTAAACGCTATTTTGCGCAAAGCGATGTTGGAAGACATCGACAAGGCGCACGGGTAG