Detailed information of TA system TA091948

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 3812943..3813571
• Replicon: chromosome
• Accession: NZ_CP025757
• Organism: Citrobacter freundii complex sp. CFNIH2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: WM46_RS18720
• Protein ID: WP_102603016.1
• Coordinates: 3812943..3813329 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: WM46_RS18725
• Protein ID: WP_161215612.1
• Coordinates: 3813329..3813571 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM46_RS18700	3808296..3809633	+	1338	WP_102603012.1	VWA domain-containing protein	-
WM46_RS18705	3809651..3811147	+	1497	WP_102603013.1	HAMP domain-containing histidine kinase	-
WM46_RS18710	3811152..3811838	+	687	WP_102603014.1	response regulator transcription factor	-
WM46_RS18715	3811983..3812912	+	930	WP_102603015.1	formate dehydrogenase accessory protein FdhE	-
WM46_RS18720	3812943..3813329	-	387	WP_102603016.1	type II toxin-antitoxin system VapC family toxin	Toxin
WM46_RS18725	3813329..3813571	-	243	WP_161215612.1	ribbon-helix-helix domain-containing protein	Antitoxin
WM46_RS18730	3813779..3814687	+	909	WP_102603017.1	alpha/beta hydrolase	-
WM46_RS18735	3814752..3814994	+	243	WP_102603018.1	type II toxin-antitoxin system ParD family antitoxin	-
WM46_RS18740	3814987..3815274	+	288	WP_102603019.1	type II toxin-antitoxin system RelE/ParE family toxin	-
WM46_RS18745	3815278..3816219	-	942	WP_042325456.1	fatty acid biosynthesis protein FabY	-
WM46_RS18750	3816264..3816701	-	438	WP_102603020.1	D-tyrosyl-tRNA(Tyr) deacylase	-
WM46_RS18755	3816698..3817570	-	873	WP_044257165.1	virulence factor BrkB family protein	-
WM46_RS18760	3817564..3818163	-	600	WP_061075715.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14424.71 Da
• Isoelectric Point: 9.4120

>T91948 WP_102603016.1 NZ_CP025757:c3813329-3812943 [Citrobacter freundii complex sp. CFNIH2]
MQKGPVLFDTNILIDLFSGHQEAQRVLEAYPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSLEIAERSVNL
RQEYGIKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQTGR

Nucleotide

• Length: 387 bp

>T91948 NZ_CP025757:c3813329-3812943 [Citrobacter freundii complex sp. CFNIH2]
ATGCAAAAGGGACCCGTGTTGTTCGATACCAATATCCTGATTGATTTATTTAGCGGGCACCAGGAAGCGCAGCGGGTACT
GGAAGCGTATCCGCCGCAGAATGCCATCAGCTTGATTACCTGGATGGAAGTCATGGTGGGGGCAAAAAAATACCATCAGG
AATATCGTACGCGTGTGGCATTGAGTGCGTTCAATATCATCGGTGTATCACTGGAAATTGCTGAGCGAAGCGTGAATCTC
AGGCAGGAATATGGGATAAAACTTCCGGACGCGATCATACTGGCTACCGCGCAGATTCATCGGTTTGCGCTTGTGACGCG
CAACACCAGAGATTTTGCAGGGATCCCTGGCGTCATTACCCCTTATCAGTTGCAAACCGGACGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT91948 WP_161215612.1 NZ_CP025757:c3813571-3813329 [Citrobacter freundii complex sp. CFNIH2]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT91948 NZ_CP025757:c3813571-3813329 [Citrobacter freundii complex sp. CFNIH2]
ATAATGGCTGGAATGGACATGGGCAGAATATTACTCGACTTATCCGATGAGGTGATCAAACGCCTTGATGATCTCAAGCA
GCAGCGGAATCTGCCCCGTGCGGAGTTGCTGCGCGAAGCGGTTGAACAGTACCTTGAAAGGCAAGGCCAGGCTGAAACCA
CAATTTCCAATGCGCTGGGGCTCTGGCAGGGTTGTGAGGAAGACGGCGTTGAGTATCAGAGGACGCTGCGCGAGGAATGG
TAA