Detailed information of TA system TA091947

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3261446..3262214
• Replicon: chromosome
• Accession: NZ_CP025757
• Organism: Citrobacter freundii complex sp. CFNIH2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: WM46_RS15880
• Protein ID: WP_102602791.1
• Coordinates: 3261828..3262214 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: WM46_RS15875
• Protein ID: WP_187394507.1
• Coordinates: 3261446..3261772 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM46_RS15835	3257537..3257938	+	402	WP_048242038.1	hypothetical protein	-
WM46_RS15840	3258035..3258502	+	468	WP_102602784.1	hypothetical protein	-
WM46_RS15845	3258693..3258944	+	252	WP_102602785.1	DUF905 family protein	-
WM46_RS15850	3259061..3259885	+	825	WP_102602786.1	DUF945 domain-containing protein	-
WM46_RS15855	3259909..3260139	+	231	WP_102602787.1	DUF905 domain-containing protein	-
WM46_RS15860	3260219..3260662	+	444	WP_044349771.1	antirestriction protein	-
WM46_RS15865	3260674..3261153	+	480	WP_102602788.1	DNA repair protein RadC	-
WM46_RS15870	3261167..3261388	+	222	WP_102602789.1	DUF987 domain-containing protein	-
WM46_RS15875	3261446..3261772	+	327	WP_187394507.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
WM46_RS15880	3261828..3262214	+	387	WP_102602791.1	TA system toxin CbtA family protein	Toxin
WM46_RS15885	3262301..3263149	+	849	WP_102602792.1	DUF4942 domain-containing protein	-
WM46_RS15890	3263625..3265484	-	1860	WP_102602793.1	bifunctional glutathionylspermidine amidase/synthase	-
WM46_RS15895	3265680..3266549	+	870	WP_102602794.1	glutathione-dependent disulfide-bond oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (7-110)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14264.37 Da
• Isoelectric Point: 8.0799

>T91947 WP_102602791.1 NZ_CP025757:3261828-3262214 [Citrobacter freundii complex sp. CFNIH2]
MQTTLPFMRAVSSCPSPVGVWQTLLTSLLAHHYGLTLNDTPFSDEQVIQQHIEAGISLADALNFIVEKYELVRTDRPGFS
ILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPSKR

Nucleotide

• Length: 387 bp

>T91947 NZ_CP025757:3261828-3262214 [Citrobacter freundii complex sp. CFNIH2]
ATGCAAACTACATTGCCCTTCATGCGGGCGGTATCGTCATGTCCGTCTCCCGTGGGTGTCTGGCAGACCCTGCTGACCAG
CCTGCTGGCACACCACTATGGTCTCACGCTTAACGATACGCCGTTCAGTGATGAACAGGTCATTCAGCAGCATATCGAGG
CGGGTATCTCGCTGGCCGATGCCCTGAATTTTATTGTAGAAAAGTACGAACTGGTCCGGACTGACCGGCCAGGCTTCAGC
ATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGACAC
GTATAAAGAGGTGACGGCGATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAAGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12034.72 Da
• Isoelectric Point: 6.9457

>AT91947 WP_187394507.1 NZ_CP025757:3261446-3261772 [Citrobacter freundii complex sp. CFNIH2]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANFDGQFCDADLRHLDQAFPVLMKQLGLMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPAATA

Nucleotide

• Length: 327 bp

>AT91947 NZ_CP025757:3261446-3261772 [Citrobacter freundii complex sp. CFNIH2]
ATCAGCGAACCCTGGTGGGGACTCAGGCGCAGTGTTTCCCCGTGTTTTGGTGCCCGCCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGGGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACACTGTATGCA
AAAGGTCTGACCTGCGAGGCCGATACGCTCGGTTCGCACGGTTATGTTTATATCGCGATTTATCCCACTCCTGCCGCCAC
GGCATAA