Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2522677..2523297 Replicon chromosome
Accession NZ_CP025757
Organism Citrobacter freundii complex sp. CFNIH2

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag WM46_RS12450 Protein ID WP_001280991.1
Coordinates 2523079..2523297 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag WM46_RS12445 Protein ID WP_042324455.1
Coordinates 2522677..2523051 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM46_RS12435 2517799..2518992 + 1194 WP_061076661.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
WM46_RS12440 2519015..2522164 + 3150 WP_061076660.1 efflux RND transporter permease AcrB -
WM46_RS12445 2522677..2523051 + 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
WM46_RS12450 2523079..2523297 + 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
WM46_RS12455 2523474..2524025 + 552 WP_102602414.1 maltose O-acetyltransferase -
WM46_RS12460 2524139..2524609 + 471 WP_102602415.1 YlaC family protein -
WM46_RS12465 2524659..2524799 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
WM46_RS12470 2525022..2526572 + 1551 WP_061076657.1 EAL domain-containing protein -
WM46_RS12475 2526612..2526965 - 354 WP_061076656.1 DUF1428 family protein -
WM46_RS12480 2527048..2527644 - 597 WP_102602416.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T91946 WP_001280991.1 NZ_CP025757:2523079-2523297 [Citrobacter freundii complex sp. CFNIH2]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T91946 NZ_CP025757:2523079-2523297 [Citrobacter freundii complex sp. CFNIH2]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTGCGACGCTGTCAGACAATTGACACGCTTGAGCGCGT
GATCGAGAAAAATAAATATGAATTATCTGACAATGAACTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTCGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT91946 WP_042324455.1 NZ_CP025757:2522677-2523051 [Citrobacter freundii complex sp. CFNIH2]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT91946 NZ_CP025757:2522677-2523051 [Citrobacter freundii complex sp. CFNIH2]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGCTGCTTTGTGAATGCCACCAGAGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References