Detailed information of TA system TA091943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1287575..1288211
• Replicon: chromosome
• Accession: NZ_CP025757
• Organism: Citrobacter freundii complex sp. CFNIH2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2K9P8U1
• Locus tag: WM46_RS06540
• Protein ID: WP_072015688.1
• Coordinates: 1287575..1287766 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: WM46_RS06545
• Protein ID: WP_187394497.1
• Coordinates: 1287795..1288211 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM46_RS06520	1282765..1284726	+	1962	WP_102601774.1	U32 family peptidase	-
WM46_RS06525	1284767..1284895	-	129	WP_071887674.1	DUF2474 domain-containing protein	-
WM46_RS06530	1284895..1285905	-	1011	WP_102601775.1	cytochrome d ubiquinol oxidase subunit II	-
WM46_RS06535	1285905..1287308	-	1404	WP_102601776.1	cytochrome ubiquinol oxidase subunit I	-
WM46_RS06540	1287575..1287766	+	192	WP_072015688.1	type II toxin-antitoxin system HicA family toxin	Toxin
WM46_RS06545	1287795..1288211	+	417	WP_187394497.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
WM46_RS06550	1288298..1289707	+	1410	WP_102601778.1	PLP-dependent aminotransferase family protein	-
WM46_RS06555	1290032..1291177	+	1146	WP_102601779.1	ABC transporter substrate-binding protein	-
WM46_RS06560	1291195..1292208	+	1014	WP_102601780.1	ABC transporter ATP-binding protein	-
WM46_RS06565	1292209..1293153	+	945	WP_102601781.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7177.36 Da
• Isoelectric Point: 11.6208

>T91943 WP_072015688.1 NZ_CP025757:1287575-1287766 [Citrobacter freundii complex sp. CFNIH2]
VKQSEFRRWLESQGVGVTNGSHHLKLRYRGKRSVMPRHPGDEIKEALRKAILKQLGLSPSSLI

Nucleotide

• Length: 192 bp

>T91943 NZ_CP025757:1287575-1287766 [Citrobacter freundii complex sp. CFNIH2]
GTGAAGCAGAGCGAATTCAGACGCTGGCTGGAATCTCAGGGAGTCGGGGTAACGAATGGATCGCACCATTTGAAGCTCAG
ATATCGCGGAAAACGCAGCGTCATGCCCAGACATCCGGGTGATGAGATTAAAGAGGCCTTGCGTAAAGCCATTTTAAAGC
AATTAGGCCTGTCGCCATCATCACTGATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15202.40 Da
• Isoelectric Point: 4.9371

>AT91943 WP_187394497.1 NZ_CP025757:1287795-1288211 [Citrobacter freundii complex sp. CFNIH2]
MRYPVRLVSAPEGGYVVSFPDIPEALTQGETRQQSLDTALEALVTAFEFYFEDNQSVPLPSPVKEGDDFVEVPLSVASKV
LLLNAFLASKISQQELAKRIGRPKQEITRLFDLRHATKIDAVQTAARALGKELSLTLS

Nucleotide

• Length: 417 bp

>AT91943 NZ_CP025757:1287795-1288211 [Citrobacter freundii complex sp. CFNIH2]
ATGCGCTACCCCGTTCGTCTCGTTTCTGCGCCAGAAGGTGGCTATGTGGTTTCGTTTCCGGATATTCCGGAAGCGCTCAC
CCAGGGGGAGACCCGCCAGCAATCCCTTGATACCGCACTGGAAGCGTTGGTCACCGCCTTTGAATTCTATTTCGAAGATA
ACCAGTCCGTCCCGTTACCTTCGCCGGTGAAAGAGGGCGATGACTTTGTTGAAGTACCGCTCAGCGTCGCGTCAAAAGTC
CTTTTACTCAACGCTTTTCTGGCATCAAAAATCAGCCAGCAGGAGCTGGCGAAACGCATCGGCCGACCGAAACAGGAAAT
TACCCGTCTGTTCGACTTGCGGCATGCAACAAAAATTGATGCTGTGCAGACGGCGGCGCGTGCGCTCGGCAAAGAACTGT
CACTTACGTTGTCGTAA

Structures

Toxin

• AlphaFold DB: A0A2K9P8U1