Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 144042..144649 Replicon plasmid pEFA-99d7
Accession NZ_CP025756
Organism Enterococcus faecium strain AALTL

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag BXT96_RS15525 Protein ID WP_002321032.1
Coordinates 144299..144649 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag BXT96_RS15520 Protein ID WP_002287514.1
Coordinates 144042..144305 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BXT96_RS15480 139392..139865 + 474 WP_002321302.1 single-stranded DNA-binding protein -
BXT96_RS15485 140231..140491 + 261 WP_002321303.1 hypothetical protein -
BXT96_RS15490 140936..141142 + 207 WP_002296239.1 hypothetical protein -
BXT96_RS15495 141142..141393 + 252 WP_002305766.1 hypothetical protein -
BXT96_RS15500 141409..141825 + 417 WP_002305764.1 hypothetical protein -
BXT96_RS15505 141807..142079 + 273 WP_002348912.1 hypothetical protein -
BXT96_RS15510 142246..142647 + 402 WP_002305759.1 IS200/IS605 family transposase -
BXT96_RS15515 142659..143804 + 1146 WP_002305757.1 IS200/IS605 family element transposase accessory protein TnpB -
BXT96_RS15520 144042..144305 + 264 WP_002287514.1 hypothetical protein Antitoxin
BXT96_RS15525 144299..144649 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BXT96_RS15530 144673..145287 - 615 WP_002318230.1 recombinase family protein -
BXT96_RS15535 145601..146023 + 423 WP_002288787.1 hypothetical protein -
BXT96_RS15540 146033..146236 + 204 WP_002299573.1 hypothetical protein -
BXT96_RS15545 146447..147031 + 585 WP_002299575.1 recombinase family protein -
BXT96_RS15550 147200..148525 + 1326 WP_002303115.1 Y-family DNA polymerase -
BXT96_RS15555 148518..148868 + 351 WP_002303114.1 hypothetical protein -
BXT96_RS15560 148865..149044 + 180 WP_002322386.1 hypothetical protein -
BXT96_RS15565 149056..149349 + 294 WP_002322251.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..258454 258454
- inside IScluster/Tn - - 142246..147031 4785


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T91934 WP_002321032.1 NZ_CP025756:144299-144649 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T91934 NZ_CP025756:144299-144649 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT91934 WP_002287514.1 NZ_CP025756:144042-144305 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT91934 NZ_CP025756:144042-144305 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References