Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 55065..55709 Replicon plasmid pCV839-06-p2
Accession NZ_CP025752
Organism Escherichia coli strain CV839-06

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag CV83906_RS01005 Protein ID WP_000833471.1
Coordinates 55065..55247 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag CV83906_RS01010 Protein ID WP_000466317.1
Coordinates 55272..55709 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83906_RS00970 50395..51198 - 804 WP_000210482.1 replication initiation protein -
CV83906_RS00985 52439..53626 - 1188 WP_000937614.1 IS91-like element IS91 family transposase -
CV83906_RS00990 53626..53991 - 366 WP_000124098.1 hypothetical protein -
CV83906_RS00995 54083..54265 + 183 WP_000449470.1 hypothetical protein -
CV83906_RS01000 54591..54869 + 279 WP_000545935.1 cobalamin biosynthesis protein CbiX -
CV83906_RS01005 55065..55247 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
CV83906_RS01010 55272..55709 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CV83906_RS01015 55738..56442 - 705 WP_001279864.1 hypothetical protein -
CV83906_RS01020 56439..57200 - 762 WP_001097938.1 conjugal transfer protein TraL -
CV83906_RS01025 57210..57617 - 408 WP_001190622.1 hypothetical protein -
CV83906_RS01030 57951..58286 + 336 WP_000963832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC 1..63821 63821
- flank IS/Tn - - 52439..53626 1187


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T91906 WP_000833471.1 NZ_CP025752:55065-55247 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T91906 NZ_CP025752:55065-55247 [Escherichia coli]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT91906 WP_000466317.1 NZ_CP025752:55272-55709 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT91906 NZ_CP025752:55272-55709 [Escherichia coli]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGTTCTTATTCTGTTGATGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTCAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAGCTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References