Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3042608..3043439 Replicon chromosome
Accession NZ_CP025747
Organism Escherichia coli strain ML35

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag C1I23_RS16470 Protein ID WP_000854814.1
Coordinates 3043065..3043439 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag C1I23_RS16465 Protein ID WP_001285585.1
Coordinates 3042608..3042976 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1I23_RS16430 3039516..3039971 + 456 WP_000581504.1 hypothetical protein -
C1I23_RS16435 3040050..3040283 + 234 WP_001119729.1 DUF905 family protein -
C1I23_RS16445 3040383..3041201 + 819 WP_001234629.1 DUF945 domain-containing protein -
C1I23_RS16450 3041283..3041762 + 480 WP_000860087.1 antirestriction protein -
C1I23_RS16455 3041774..3042250 + 477 WP_001186726.1 RadC family protein -
C1I23_RS16460 3042313..3042534 + 222 WP_000692323.1 DUF987 domain-containing protein -
C1I23_RS16465 3042608..3042976 + 369 WP_001285585.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
C1I23_RS16470 3043065..3043439 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
C1I23_RS16475 3043436..3043630 + 195 WP_000988600.1 hypothetical protein -
C1I23_RS26335 3043643..3043756 + 114 WP_001161660.1 DUF957 domain-containing protein -
C1I23_RS16480 3044045..3044173 - 129 Protein_3013 transposase domain-containing protein -
C1I23_RS16485 3044245..3044427 + 183 WP_001016348.1 hypothetical protein -
C1I23_RS16490 3044528..3044857 - 330 WP_000450409.1 DUF496 family protein -
C1I23_RS16495 3045029..3046087 - 1059 WP_001200891.1 FUSC family protein -
C1I23_RS16500 3046285..3046758 - 474 WP_001105389.1 DNA gyrase inhibitor SbmC -
C1I23_RS16505 3046877..3048043 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T91894 WP_000854814.1 NZ_CP025747:3043065-3043439 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T91894 NZ_CP025747:3043065-3043439 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT91894 WP_001285585.1 NZ_CP025747:3042608-3042976 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT91894 NZ_CP025747:3042608-3042976 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References