Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 2994926..2995461 Replicon chromosome
Accession NZ_CP025747
Organism Escherichia coli strain ML35

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag C1I23_RS16185 Protein ID WP_001270415.1
Coordinates 2995174..2995461 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag C1I23_RS16180 Protein ID WP_001132890.1
Coordinates 2994926..2995177 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1I23_RS16140 2990325..2990639 + 315 WP_001303878.1 PerC family transcriptional regulator -
C1I23_RS16145 2990716..2991148 + 433 Protein_2962 T3SS effector protein NleH -
C1I23_RS16155 2991421..2992296 + 876 WP_096843318.1 secretion protein EspS -
C1I23_RS16160 2992385..2993598 + 1214 Protein_2964 IS3 family transposase -
C1I23_RS16165 2993657..2994264 + 608 Protein_2965 transposase -
C1I23_RS16175 2994542..2994754 + 213 WP_000402944.1 hypothetical protein -
C1I23_RS16180 2994926..2995177 + 252 WP_001132890.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C1I23_RS16185 2995174..2995461 + 288 WP_001270415.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C1I23_RS16190 2995740..2996720 + 981 WP_000019448.1 IS5-like element ISKpn26 family transposase -
C1I23_RS16200 2997194..2997496 + 303 WP_032146770.1 hypothetical protein -
C1I23_RS16205 2997516..2997840 + 325 Protein_2971 hypothetical protein -
C1I23_RS16210 2997911..2998189 - 279 Protein_2972 transposase -
C1I23_RS16220 2998420..2999109 - 690 Protein_2973 IS66 family transposase -
C1I23_RS16225 2999461..3000051 - 591 WP_032258008.1 bfpT-regulated chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleA/espI 2961769..3028812 67043
- inside IScluster/Tn - - 2984832..2999148 14316


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11163.21 Da        Isoelectric Point: 10.4350

>T91893 WP_001270415.1 NZ_CP025747:2995174-2995461 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQHFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVCQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T91893 NZ_CP025747:2995174-2995461 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACATTTTGC
GAAAAAGCTCAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTGTCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.53 Da        Isoelectric Point: 4.2108

>AT91893 WP_001132890.1 NZ_CP025747:2994926-2995177 [Escherichia coli]
MPNIILCDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT91893 NZ_CP025747:2994926-2995177 [Escherichia coli]
ATGCCTAACATTATTCTCTGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCTACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTATACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References