Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2391602..2392240 Replicon chromosome
Accession NZ_CP025747
Organism Escherichia coli strain ML35

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C1I23_RS12915 Protein ID WP_000813794.1
Coordinates 2391602..2391778 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C1I23_RS12920 Protein ID WP_001270286.1
Coordinates 2391824..2392240 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1I23_RS12895 2387221..2388435 - 1215 WP_001326689.1 BenE family transporter YdcO -
C1I23_RS12900 2388488..2389024 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C1I23_RS12905 2389097..2391058 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C1I23_RS12910 2391150..2391380 - 231 WP_000494244.1 YncJ family protein -
C1I23_RS12915 2391602..2391778 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C1I23_RS12920 2391824..2392240 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C1I23_RS12925 2392319..2393725 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
C1I23_RS12930 2393970..2395115 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
C1I23_RS12935 2395133..2396146 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C1I23_RS12940 2396147..2397088 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T91878 WP_000813794.1 NZ_CP025747:2391602-2391778 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T91878 NZ_CP025747:2391602-2391778 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT91878 WP_001270286.1 NZ_CP025747:2391824-2392240 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT91878 NZ_CP025747:2391824-2392240 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References