Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1181997..1182615 Replicon chromosome
Accession NZ_CP025747
Organism Escherichia coli strain ML35

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C1I23_RS06160 Protein ID WP_001291435.1
Coordinates 1181997..1182215 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C1I23_RS06165 Protein ID WP_000344800.1
Coordinates 1182241..1182615 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1I23_RS06130 1177379..1177732 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C1I23_RS06135 1177774..1179324 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C1I23_RS06140 1179488..1179958 - 471 WP_000136192.1 YlaC family protein -
C1I23_RS06145 1180074..1180625 - 552 WP_000102564.1 maltose O-acetyltransferase -
C1I23_RS06150 1180734..1181714 + 981 WP_000019448.1 IS5-like element ISKpn26 family transposase -
C1I23_RS06160 1181997..1182215 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C1I23_RS06165 1182241..1182615 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C1I23_RS06170 1183160..1186309 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C1I23_RS06175 1186332..1187525 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1180734..1181714 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T91867 WP_001291435.1 NZ_CP025747:c1182215-1181997 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T91867 NZ_CP025747:c1182215-1181997 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT91867 WP_000344800.1 NZ_CP025747:c1182615-1182241 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT91867 NZ_CP025747:c1182615-1182241 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References