Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 175395..175996 Replicon plasmid pYDC107_184
Accession NZ_CP025708
Organism Escherichia coli strain YDC107

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag YDC107_RS26540 Protein ID WP_001216034.1
Coordinates 175616..175996 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag YDC107_RS26535 Protein ID WP_001190712.1
Coordinates 175395..175616 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YDC107_RS26525 172415..173659 - 1245 WP_105955882.1 restriction endonuclease subunit S -
YDC107_RS26530 173656..175212 - 1557 WP_001553856.1 type I restriction-modification system subunit M -
YDC107_RS26535 175395..175616 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
YDC107_RS26540 175616..175996 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
YDC107_RS26545 176001..176180 + 180 WP_001513661.1 hypothetical protein -
YDC107_RS26550 176208..176567 + 360 WP_001513660.1 hypothetical protein -
YDC107_RS26555 176491..176904 + 414 Protein_238 DDE-type integrase/transposase/recombinase -
YDC107_RS26560 176854..177171 - 318 WP_001513659.1 hypothetical protein -
YDC107_RS26565 177399..178415 - 1017 WP_012372828.1 IS5-like element IS5 family transposase -
YDC107_RS26570 178623..180026 + 1404 WP_001373486.1 S-methylmethionine permease -
YDC107_RS26575 180013..180945 + 933 WP_000081352.1 homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..184098 184098


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T91821 WP_001216034.1 NZ_CP025708:175616-175996 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T91821 NZ_CP025708:175616-175996 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTAGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT91821 WP_001190712.1 NZ_CP025708:175395-175616 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT91821 NZ_CP025708:175395-175616 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References